Recombinant Mouse CD40 Ligand, soluble

Store lyophilized protein at -20C and reconstituted protein in working aliquots at -20C. Avoid repeated freeze-thaw cycles.

CD40, a member of the TNF receptor family, is a cell surface protein expressed on B-cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T-cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B-cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40LG leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. The soluble form of CD40LG is an 18 kDa protein comprising the entire TNF homologous region of CD40LG and is generated in vivo by an intracellular proteolytic processing of the full length CD40LG. Recombinant mouse CD40LG is a soluble protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40LG.

Centrifuge vial prior to opening. Add sterile distilled water to the vial to fully solubilize the antibody to a concentration of 0.1-1.0 mg/ml.

< 0.1 ng/ug of CD40LG