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Recombinant Mouse Leptin

Recombinant Mouse Leptin

Manufacturer	Cell Sciences
Category	Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Peptides & Proteins Recombinant Proteins Obesity Endocrine Regulation of Lipid Metabolism
Type	Proteins
Specific against	Mouse
Amount	200 ug
Host	E.coli
Item no.	CS-CRL301A
Targets	LEP
eClass 6.1	34160400
eClass 9.0	42020190
Available
Biological Activity	The activity is measured in a cell proliferation assay using BaF3 cells and is typically 0.2-1 ng/mL.
Description	Leptin a protein that is thought to have a critical role in the physiologic regulation of body weight via its capacity to inhibit food intake and stimulate energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant mouse Leptin is a non-glycosylated protein, containing 147 amino acids.
Endotoxin Level	Measured by kinetic LAL analysis and is typically <= 1 EU/ug protein.
Formulation	Recombinant mouse Leptin is lyophilized from a concentrated (1mg/mL) solution of 0.1% TFA.
Molecular Weight	16.1 kDa
Reconstitution	Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 20 mM HCl at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
Storage and Stability	Lyophilized product is very stable at -20C. Reconstituted material should be aliquoted and frozen at -20C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.