Recombinant Staphylococcal Protein-A

Store in working aliquots below -20C. Avoid repeated freeze-thaw cycles.

Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. It couples to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing. The recombinant protein A is produced by expressing a modified protein A gene in, E. coli, . A specific purification process with strict quality control was taken to get the recombinant protein A with the purity of more than 98%. No human IgG affinity step is used during validated fermentation and purification and the protein is devoid of bacterial contaminant found normally in native Protein A (free of Staphylococcus endotoxins and hemolysin).