Recombinant Saccharomyces cerevisiae Heat Shock Protein 104

Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

HSP104 is a molecular chaperone required for stress tolerance and for maintenance of [psi(+)] prions in the budding yeast, Saccharomyces cerevisiae . HSP104 can protect yeast cells against high temperature and high concentration of ethanol but mutation studies have shown this protein is not required for normal growth. HSP104 was cloned into an, E. coli, expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. Recombinant HSP104 produced in, E. coli, is a single, non-glycosylated polypeptide chain containing 908 amino acids