Horseradish Peroxidase, HRP

The enzyme horseradish peroxidase, found in horseradish, is used extensively in molecular biology and in antibody amplification and detection, among other things. For example, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase (HRP) has become a major tool. In its brief history, this method has probably been used by more neurobiologists than have used the Golgi stain since its discovery in 1870." Horseradish peroxidase is also highly used in techniques such as Western blot and ELISAs. HRP is widely used as an enzymatic label in immunoassays. Usually, the enzyme is coupled to antibodies, lectins or haptens. Coupling to antibodies etc. may be performed through the carbohydrate side chains of the HRP. HRP consists of the basic isoenzyme having a molecular weight of 44 kDa. The Horseradish Peroxidase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.

It is recommended to reconstitute the lyophilized HRP in sterile 18MU-cm water not less than 100 ug/ml or more than 10 mg/ml solutions.