Recombinant Tobacco Etch Virus Protease GST

Biomolecules

Recombinant TEV Protease (rTEV) is a site-specific protease purified from, E. coli, by the affinity tag, GST tag. The protease can be used for the removal of affinity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30C, however, the enzyme can be used at temperatures as low as 4C. Following digestion, TEV Protease can be removed from the reaction via the GST tag sequence by affinity chromatography.

One unit of rTEV cleaves 85% of 3 ug control substrate in 1 h at 30C.