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Native Porcine Pancreatic Elastase-1, High Purity

Native Porcine Pancreatic Elastase-1, High Purity

Manufacturer	Cell Sciences
Category	Oncology Peptides & Proteins Cardiovascular Research Atherosclerosis Enzymes By Organ Pancreatic Cancer
Type	Proteins
Specific against	Pig
Amount	1000 Units
Host	Porcine
Item no.	CS-CRP262B
Targets	CELA1
eClass 6.1	34160400
eClass 9.0	42020190
Available
Description	Native Pig Pancreatic Elastase
Formulation	Lyophilized from a salt free buffer. Free of trypsin, chymotrypsin, lipase, alpha-amylase, and the carboxypeptidases. This high purity elastase is isolated from 2X crystallized elastase. The 2X elastase is chromatographed on DEAE-cellulose at pH 8.8 to remove anionic non-specific protein. The elastolytic effluent is chromatographed at pH 5.0 on CM-cellulose recovering the highest specific activity by elution with salt gradient. Trace trypsin, and chymotrypsin contamination is removed by affinity chromatography.
Molecular Weight	25.5 kDa
Reconstitution	Centrifuge vial prior to opening . Reconstitute in sterile distilled water.
Storage and Stability	Stable for 1 year at 2-4C.
Specificity	115-125 units/mg of protein on the substrate elastin-orcein. One unit will solubilize 1 mg of elastin in 20 minutes at pH 8.8 and 37C. > 9 units/mg of protein on the substrate Suc-Ala-Ala-Ala-pNA. One unit will hydrolyze 1 umole per minute at pH 8.3 and 25C.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.