Synthetic CK1 Substrate (RRKDLHDDEEDEAMSITA) Peptide

Quality guaranteed for 12 months Store at -80C Avoid freeze / thaw cycles

The synthetic peptide RRKDLHDDEEDEAMSITA can be used as a substrate for CK1 (casein kinase 1) in, in vitro, kinase assays. It is phosphorylated by CK1 with a Km of 172 uM. In mammals and vertebrates, there are seven CK1 genes coding for a, b, g1, g2, g3, d, and e isoforms, and some of these genes generate several proteins through alternative splicing. The CK1 isoforms may have different functions, for instance, CK1e is involved in regulating circadian rhythm through phosphoration of period (per) proteins, and it has also been linked to the activity of group I metabotropic glutamate receptors. CK1d may be involved in neurodegenerative diseases. CK1a apparently participates in the phosphorylation of G protein regulated receptors and nuclear factor for activated T cells 4 (NF-AT4) phosphorylation. However, in vitro, studies with model peptides using different isoforms of CK1 have not shown significant differences in the preferred target sequences. Differences in functions of isoforms most probably involve differential expression and cellular localization and specific docking sites that confer substrate selectivity. CK1e and CK1d have an interesting regulatory mechanism where autophosphorylation of their carboxyl-terminal extensions inhibits their catalytic activity. Dephosphorylation of these carboxyl residues by calcineurin may be a mechanism for regulating the activity of these isoforms.