MB49 cells

Disease:

Urothelial carcinoma

Origin:

MB49 cells are derived from C57BL/Icrf-a? mouse bladder epithelial cells that were transformed by a single 24-hour treatment with the chemical carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) on the second day of a long term primary culture. Transformed cells transplanted into syngeneic mice were shown to generate carcinomas . While of male origin, karyotype analyses indicate the loss of the Y chromosome in 100% of the cells analyzed . This abnormality is a frequent early event in human bladder cancer.

Species:

Mus musculus, transgenic mouse

Tissue:

Bladder

Morphology:

Epithelial

Properties:

Adherent

Strain:

C57BL/6

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:6 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. This cell line will not become confluent. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO