CCRF-CEM cells

Disease:
Acute lymphoblastic leukemia
Origin:
Lymphoblast
Species:
Homo sapiens
Tissue:
Blood
Morphology:
Lymphoblast
Properties:
Suspension
Cytogenic data:
CCRF-CEM cells were derived from the peripheral blood buffy coat of a child (CEM) with acute lymphoblastic leukemia who had originally presented with lymphosarcoma
Patient:
Female, Caucasian, 4 yrs of age
Medium:
RPMI1640 + 10% FBS
Subculture:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10⁵ viable cells/ml. Maintain cell density between 1 to 2 X 10⁶ viable cells/ml.
Medium Renewal:
Add fresh medium (20% to 30% by volume) every 2 to 3 days.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level: I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Isoenzymes: ADA, 1; ES-D, 1; G6PD, B ; GLO-I, 1; PEP-D, 1; PGD, C; PGM1, 1 ; PGM3, 0
DNA Profile (STR): Amelogenin: X ; CSF1PO: 10, 11; D13S317: 11, 12; D16S539: 10, 13; D5S818: 12, 13; D7S820: 9, 13; THO1: 6, 7; TPOX: 8; vWA: 17, 19
Related cells by Organ: CCRF-CEM K562
LP-1
MOLM-13
RPMI-8226
U937
RAW 264.7

Cell Lines