F-36P cells

Disease:

Acute myeloid leukemia, myelodysplastic syndrome

Origin:

Established at diagnosis of a 68-year-old male with acute myeloid leukemia (AML M6) secondary to myelodysplastic syndrome (MDS, subtype refractory anemia with excess of blasts, RAEB) in 1989; cells were described to be proliferatively responsive to GM-CSF and IL-3. Cells have been shown to synthesize haemoglobin when GM-CSF or IL-3 is substituted by erythropoietin (Epo). They have been reported to be positive for leukocyte common antigen (CD45) and some multilineage markers such as CD13, CD33 and CD34, but are negative for T- and B-cell antigens and mature myelomonocytic antigens. Due to their reaction with some monoclonal antibodies recognising erythroid and platelet glycoproteins it has been suggested that F-36P has a multilineage phenotype.

Species:

Homo sapiens

Tissue:

Pleural effusion

Morphology:

Lymphoblastoid

Properties:

Polymorph cells growing as adherent cells and singly in suspension

Cytogenic data:

human flat-moded hypodiploid karyotype with 6% polyploidy - 41(40-43)< 2n> XY, -3, -13, -16, -19, -21, -22, +2mar, ins(X; 13)(p11; q?q?), del(3)(p13p24), add(5)(q11), dup(5)(q11q35), der(6)add(6)(p23)dup(6)(q25q27), der(7)add(7)(p13)add(7)(q12), add(9)(q35), der(9)del(9)(q11q34)inv(9)(p2?4; q34), der(10)t(8; 10)(q24; p14)t(10; 7)(q25; q12), del(11)(q14q21), der(21)t(10; 21)(p14; p12) - sideline with dup(1)(p35p36), dup(2)(q1?q2?), del(3)(p13p24) - matches published karyotype

Patient:

Male, 68 yrs of age

Medium:

RPMI-1640 Medium + 20% FBS + 10 ng/mL GM-CSF or 5 ng/mL rh IL-3

Subculture:

Subculture saturated culture 1:3 to 1:8 every 2-5 days; seed out at ca. 0.3-0.5 x 10^6 cells/ml; maintain at ca. 1.0 x 10^6 cells/ml; maximum density at ca. 1.8 x 10^6 cells/ml. Upon cytokine withdrawal cells do not die but undergo a stationary survival status. REMARKS: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote growth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Therefore, we cannot guarantee indefinite stability of factor-dependence of cell lines. Culture at 5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 7.5% (v/v) DMSO