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Hereditary spherocytosis
This is a HAT sensitive variant of the WIL2 human B cell line.
Homo sapiens Tissue:Spleen Morphology:Lymphoblast Properties: Suspension, singly or in clumps Patient: Male, Caucasian, 5 years of age Medium: RPMI-1640 Medium + 10% FBS Subculture:Maintain at 0.3-1.0 x 10^6 cells/ml; feed cells twice-weekly, split of ca. 1:3 to 1:5; seed out at ca. 1 x 10^5 cells/ml. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: II [contain Epstein-Barr virus] Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
Spleen Morphology:Lymphoblast Properties: Suspension, singly or in clumps Patient: Male, Caucasian, 5 years of age Medium: RPMI-1640 Medium + 10% FBS Subculture:Maintain at 0.3-1.0 x 10^6 cells/ml; feed cells twice-weekly, split of ca. 1:3 to 1:5; seed out at ca. 1 x 10^5 cells/ml. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: II [contain Epstein-Barr virus] Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
Lymphoblast Properties: Suspension, singly or in clumps Patient: Male, Caucasian, 5 years of age Medium: RPMI-1640 Medium + 10% FBS Subculture:Maintain at 0.3-1.0 x 10^6 cells/ml; feed cells twice-weekly, split of ca. 1:3 to 1:5; seed out at ca. 1 x 10^5 cells/ml. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: II [contain Epstein-Barr virus] Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
Suspension, singly or in clumps Patient: Male, Caucasian, 5 years of age Medium: RPMI-1640 Medium + 10% FBS Subculture:Maintain at 0.3-1.0 x 10^6 cells/ml; feed cells twice-weekly, split of ca. 1:3 to 1:5; seed out at ca. 1 x 10^5 cells/ml. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: II [contain Epstein-Barr virus] Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
Male, Caucasian, 5 years of age Medium: RPMI-1640 Medium + 10% FBS Subculture:Maintain at 0.3-1.0 x 10^6 cells/ml; feed cells twice-weekly, split of ca. 1:3 to 1:5; seed out at ca. 1 x 10^5 cells/ml. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: II [contain Epstein-Barr virus] Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
RPMI-1640 Medium + 10% FBS Subculture:Maintain at 0.3-1.0 x 10^6 cells/ml; feed cells twice-weekly, split of ca. 1:3 to 1:5; seed out at ca. 1 x 10^5 cells/ml. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: II [contain Epstein-Barr virus] Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
Maintain at 0.3-1.0 x 10^6 cells/ml; feed cells twice-weekly, split of ca. 1:3 to 1:5; seed out at ca. 1 x 10^5 cells/ml. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 10% (v/v) DMSO Biosafety Level: II [contain Epstein-Barr virus] Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
Complete culture medium supplemented with 10% (v/v) DMSO
II [contain Epstein-Barr virus] Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
Hepatitis C: Negative DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20
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