WIL2-S cells

Disease:
Hereditary spherocytosis
Origin:
This is a HAT sensitive variant of the WIL2 human B cell line.
Species:
Homo sapiens
Tissue:
Spleen
Morphology:
Lymphoblast
Properties:
Suspension, singly or in clumps
Patient:
Male, Caucasian, 5 years of age
Medium:
RPMI-1640 Medium + 10% FBS
Subculture:
Maintain at 0.3-1.0 x 10^6 cells/ml; feed cells twice-weekly, split of ca. 1:3 to 1:5; seed out at ca. 1 x 10^5 cells/ml. Culture at 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 10% (v/v) DMSO
Biosafety Level:
II [contain Epstein-Barr virus]
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 11; D16S539: 11, 12; D5S818: 12, 13; D7S820: 9, 11; F13A01: 6, 7 F13B: 10 FESFPS: 11, 12 LPL: 9, 10 THO1: 8, 9.3; TPOX: 8, 11; vWA: 17, 20

Cell Lines