SU-DHL-1 cells

Disease:
Large cell lymphoma; diffuse histiocytic lymphoma
Origin:
The SU-DHL-1 cell line was derived derived from Metastatic Site: peritoneal effusion
Species:
Human, Homo sapiens
Tissue:
Lymph node
Morphology:
Lymphoblast-like; histiocytic
Properties:
Suspension
Cytogenic data:
88 chromosomes; t(2; 5)(p23; q35) translocation
Patient:
Male, Caucasian, 10 years of age
Medium:
AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS
Subculture:
Cultures can be maintained by the addition of fresh medium. An inoculum of 8.0 x 10^4 to 2.0 x 10^5cells/mL is recommended. Subculture when the cell concentration is between 1.0 x 10^6 and 1.5 x 10^6 cells/mL. A subcultivation ratio of 1:4 to 1:12 is recommended. Culture at 5% CO2, 37C. Feed cells once every 2 to 3 days.
Freezing Medium:
Complete culture medium supplemented with 20% FBS and 10% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 12
D13S317: 9, 13
D16S539: 11, 12
D5S818: 11, 12
D7S820: 10, 13
THO1: 6, 7
TPOX: 8
vWA: 15, 17

Cell Lines