MC116 cells

Disease:
Undifferentiated lymphoma
Origin:
Derived from the pleural effusion of a patient with undifferentiated lymphoma. The cells are EBNA negative, express surface IgM Lambda and secrete pentameric IgM which does not bind to protein A. Complement and Fc receptors were not detected.
Species:
Homo sapiens
Tissue:
Ascites
Morphology:
Lymphoblast
Properties:
Suspension
Cytogenic data:
45, XO/46, XY, dup1(q21-32), t(8; 14)(8pter --> 8q23 :: 14q32 --> 14qter ; 14pter --> 14q32 :: 8q23 --> 8qter), del10(a23)
Patient:
Male
Medium:
RPMI-1640 Medium + 20% FBS
Subculture:
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 3 X 11^5 viable cells/mL and maintain at or below 3 X 10^6 cells/mL. Culture at 5% CO2, 37C.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 10, 12
D13S317: 12
D16S539: 12
D5S818: 12
D7S820: 10, 13
THO1: 9.3, 10
TPOX: 8, 10
vWA: 15, 17

Cell Lines