MC116 cells

Disease:

Undifferentiated lymphoma

Origin:

Derived from the pleural effusion of a patient with undifferentiated lymphoma. The cells are EBNA negative, express surface IgM Lambda and secrete pentameric IgM which does not bind to protein A. Complement and Fc receptors were not detected.

Species:

Homo sapiens

Tissue:

Ascites

Morphology:

Lymphoblast

Properties:

Suspension

Cytogenic data:

45, XO/46, XY, dup1(q21-32), t(8; 14)(8pter --> 8q23 :: 14q32 --> 14qter ; 14pter --> 14q32 :: 8q23 --> 8qter), del10(a23)

Patient:

Male

Medium:

RPMI-1640 Medium + 20% FBS

Subculture:

Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 3 X 11^5 viable cells/mL and maintain at or below 3 X 10^6 cells/mL. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO