P3X63Ag8.653 cells

Disease:

Plasmacytoma; myeloma

Origin:

The cells are resistant to 8-azaguanine and are HAT sensitive. They can be used as fusion partners for producing hybridomas. The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity. Tested and found negative for ectromelia virus (mousepox).

Species:

Mus musculus, mouse

Morphology:

Lymphoblast

Properties:

Suspension

Strain:

BALB/c

Medium:

AddexBio-formulated RPMI-1640 (C0004-01) + 10% FBS

Subculture:

Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 4x10^5 viable cells/mL and subculture before the cell density reaches 2x10^6 cells/mL. Culture at 5% CO2, 37C. Renew medium once every 3 to 4 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO