P3X63Ag8.653 cells

Disease:
Plasmacytoma; myeloma
Origin:
The cells are resistant to 8-azaguanine and are HAT sensitive. They can be used as fusion partners for producing hybridomas. The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity. Tested and found negative for ectromelia virus (mousepox).
Species:
Mus musculus, mouse
Morphology:
Lymphoblast
Properties:
Suspension
Strain:
BALB/c
Medium:
AddexBio-formulated RPMI-1640 (C0004-01) + 10% FBS
Subculture:
Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 4x10^5 viable cells/mL and subculture before the cell density reaches 2x10^6 cells/mL. Culture at 5% CO2, 37C. Renew medium once every 3 to 4 days.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: P3X63Ag8.653 (AddexBio C0003028)

Cell Lines