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Adenocarcinoma
Established in 1977 from the pleural effusion of a 51-year-old black woman with metastatic adenocarcinoma of the breast; these cells carry a p53 point mutation at codon 273 leading to Arg-His substitution
Homo sapiens Tissue: Breast, mammary glands Morphology: Epithelial Properties: Adherent Cytogenic data: modal number = 64; range = 60 to 67. Patient: Female, Black, 51 yrs of age Medium: AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBS Subculture: Seed cells at 1-2 x 10^6 cells in T75 flask; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA; without CO2 (or use non-vented flask and keep the lid closed); 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
Breast, mammary glands Morphology: Epithelial Properties: Adherent Cytogenic data: modal number = 64; range = 60 to 67. Patient: Female, Black, 51 yrs of age Medium: AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBS Subculture: Seed cells at 1-2 x 10^6 cells in T75 flask; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA; without CO2 (or use non-vented flask and keep the lid closed); 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
Epithelial Properties: Adherent Cytogenic data: modal number = 64; range = 60 to 67. Patient: Female, Black, 51 yrs of age Medium: AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBS Subculture: Seed cells at 1-2 x 10^6 cells in T75 flask; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA; without CO2 (or use non-vented flask and keep the lid closed); 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
Adherent Cytogenic data: modal number = 64; range = 60 to 67. Patient: Female, Black, 51 yrs of age Medium: AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBS Subculture: Seed cells at 1-2 x 10^6 cells in T75 flask; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA; without CO2 (or use non-vented flask and keep the lid closed); 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
modal number = 64; range = 60 to 67. Patient: Female, Black, 51 yrs of age Medium: AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBS Subculture: Seed cells at 1-2 x 10^6 cells in T75 flask; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA; without CO2 (or use non-vented flask and keep the lid closed); 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
Female, Black, 51 yrs of age Medium: AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBS Subculture: Seed cells at 1-2 x 10^6 cells in T75 flask; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA; without CO2 (or use non-vented flask and keep the lid closed); 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBS Subculture: Seed cells at 1-2 x 10^6 cells in T75 flask; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA; without CO2 (or use non-vented flask and keep the lid closed); 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
Seed cells at 1-2 x 10^6 cells in T75 flask; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA; without CO2 (or use non-vented flask and keep the lid closed); 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
Complete culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2 DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
D7S820: 8 THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
THO1: 7 TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
TPOX: 8, 9 vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
vWA: 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003) Related cells by Organ: 4T1 AU-565 BT-20 BT-474 BT-483 BT-549 JIMT-1 MCF-7 SK-BR-3 T47D ZR-75-1
4T1
AU-565
BT-20
BT-474
BT-483
BT-549
JIMT-1
MCF-7
SK-BR-3
T47D
ZR-75-1
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