MDA-MB-468 cells

Disease:
Adenocarcinoma
Origin:
Established in 1977 from the pleural effusion of a 51-year-old black woman with metastatic adenocarcinoma of the breast; these cells carry a p53 point mutation at codon 273 leading to Arg-His substitution
Species:
Homo sapiens
Tissue:
Breast, mammary glands
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
modal number = 64; range = 60 to 67.
Patient:
Female, Black, 51 yrs of age
Medium:
AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBS
Subculture:
Seed cells at 1-2 x 10^6 cells in T75 flask; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA; without CO2 (or use non-vented flask and keep the lid closed); 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Isoenzymes: AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 1-2; PGM1, 1; PGM3, 2
DNA Profile (STR):
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 12
D7S820: 8
THO1: 7
TPOX: 8, 9
vWA: 18
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-468 (AddexBio C0006003)
Related cells by Organ: 4T1
AU-565
BT-20
BT-474
BT-483
BT-549
JIMT-1
MCF-7
SK-BR-3
T47D
ZR-75-1

Cell Lines