MCF-7 cells

Disease:
Breast adenocarcinoma
Origin:
Isolated from pleural effusions of a breast cancer patient. Cells exhibit some features of differentiated mammary epithelium including estradiol synthesis and formation of domes. Cells express both the wild type and variant estrogen receptors as well as progesterone receptor.
Species:
Homo sapiens
Tissue:
Breast, mammary gland
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
2n = 46, hypertriploid to hypotetraploid.
Patient:
Female, Caucasian, 69 yrs of age
Medium:
AddexBio-formulated EMEM (C0005-01) + 0.01 mg/ml human recombinant insulin + 10% Fetal Bovine Serum (FBS).
Subculture:
1:2 to 1:6 (80-90%) using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Protein Expression: estrogen receptor
Isoenzymes: AK-1, 1; ES-D, 1-2; G6PD, B; GLO-I, 1-2; PGM1, 1-2; PGM3, 1
DNA Profile (STR):
Amelogenin: X
CSF1PO:10
D13S317:11
D16S539:11, 12
D5S818:11, 12
D7S820:8, 9
TH01:6
TPOX:9, 12
vWA:14, 15
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: MCF-7 (AddexBio C0006008) Mutations in MCF-7 Cells
Related cells by Organ: 4T1
AU-565
BT-20
BT-474
BT-483
BT-549
JIMT-1
MCF-7
SK-BR-3
T47D
ZR-75-1

Cell Lines