BT-483 cells

Disease:

Ductal Carcinoma

Origin:

This cell line was isolated from a papillary invasive ductal tumor obtained from a 23 year old, normal, menstruating, parous female patient with family history of breast cancer..

Species:

Homo sapiens

Tissue:

Mammary gland/breast

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

modal number = 72; range = 46 to 130

Patient:

Female, 23 yrs of age, Caucasian

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS + 0.01 mg/mL bovine insulin

Subculture:

Subculture at 1:2; feed cells 2 to 3 times weekly. Culture at 5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO