Disease:
Ductal Carcinoma
Origin:
This cell line was isolated from a papillary invasive ductal tumor obtained from a 23 year old, normal, menstruating, parous female patient with family history of breast cancer..
Species:
Homo sapiens Tissue:
Mammary gland/breast Morphology:
Epithelial Properties:
Adherent Cytogenic data:
modal number = 72; range = 46 to 130 Patient:
Female, 23 yrs of age, Caucasian Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS + 0.01 mg/mL bovine insulin Subculture:
Subculture at 1:2; feed cells 2 to 3 times weekly. Culture at 5% CO2; 37C Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR):
Amelogenin: X, X CSF1PO: 9, 12 D13S317: 8, 12 D16S539: 11, 12 D5S818: 11 D7S820: 9, 10 THO1: 5, 9.3 TPOX: 8, 11 vWA: 17 Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: BT-483 (AddexBio C0006013) Related cells by Organ:
4T1
AU-565
BT-20
BT-474
BT-483
BT-549
JIMT-1
MCF-7
SK-BR-3
T47D
ZR-75-1