BT-549 cells

Disease:
Ductal carcinoma
Origin:
The BT-549 line was isolated in 1978 by W.G. Coutinho and E.Y. Lasfargues. Source tissue consisted of a papillary, invasive ductal tumor which had metastasized to 3 of 7 regional lymph nodes.
Species:
Homo sapiens, human
Tissue:
mammary gland; breast
Morphology:
Epithelial. The established population is polymorphic with epithelial-like components and multinucleated giant cells. A mucin-like material was secreted into the medium.
Properties:
Adherent
Cytogenic data:
modal number = 78; range = 73 to 80. The cell line is aneuploid human female, with chromosome counts in the hypertriploid range. The X chromosomes are all abnormal. Normal chromosomes N10 and N13 are clearly under-represented, with chromosomes N2, N12, and N17 also tending to be. Chromosome N8 is over-represented with respect to the copy number of other normal chromosomes, and chromosomes N5 and N18 also usually have more copies than most chromosomes. Four marker chromosomes are found: iso(13q), 10q+, del(X)(q22:), del(11)(p11:).
Patient:
Female, Caucasian, 72 years of age.
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS + Insulin (0.023 IU/mL)
Subculture:
Split sub-confluent cultures (70-80%) at a seed ratio of 1:2 to 1:6, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 10, 12
D13S317: 11
D16S539: 8
D5S818: 11
D7S820: 9, 10
THO1: 9.3
TPOX: 8
vWA: 15
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: BT-549 (AddexBio C0006017)
Related cells by Organ: 4T1
AU-565
BT-20
BT-474
BT-483
BT-549
JIMT-1
MCF-7
SK-BR-3
T47D
ZR-75-1

Cell Lines