HCT15 cells

Disease:
Colorectal adenocarcinoma
Origin:
Dukes' type C
Species:
Homo sapiens
Tissue:
Colon
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
A quasidiploid human cell line
Patient:
Male, adult
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
Subculture:
1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Isoenzymes: ES-D, 2; G6PD, B; PEP-D, 1; PGD, A; PGM1, 1-2; PGM3, 1;
DNA Profile (STR): Amelogenin: X, Y CSF1PO: 12 D13S317: 8, 11 D16S539: 12, 13 D5S818: 13 D7S820: 10, 12 THO1: 7, 9.3 TPOX: 8, 11 vWA: 18, 19 Mutations in HCT15 Cells
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: HCT-15 (AddexBio C0009010)
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Cell Lines