LS-174T cells

Disease:
Colorectal adenocarcinoma
Origin:
Dukes' type B. A trypsinised variant of LS180. The cells have microvilli and intracytoplasmic vacuoles.
Species:
Homo sapiens
Tissue:
Colon
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Modal no. 45 (2n). The cells are tumorigenic in nude mice.
Patient:
Female, Caucasian, 58 yrs of age
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
1:3 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR): Amelogenin: X ; CSF1PO: 10, 13, 14; D13S317: 10; D16S539: 11, 13; D5S818: 11, 15; D7S820: 11; THO1: 6, 7; TPOX: 8, 9; vWA: 15, 17
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: LS-174T (AddexBio C0009013)
Related cells by Organ: Caco-2 cells
CMT-93 cells
COLO 205 cells
DLD-1 cells
HCA-7 cells
HCT-116 cells
HCT-8 cells
HCT-15 cells
HT-29 cells
LoVo cells
LS-180 cells
LS-174T cells
RKO cells
SNU-407 cells
SNU-503 cells
SW1116 cells
SW48 cells
SW480 cells
SW620 cells
T84 cells

Cell Lines