SNU-503 cells

Disease:
Rectal adenocarcinoma
Origin:
Moderately differentiated tumor cells derived from metastatic site: abdominal wall.
Species:
Homo sapiens
Tissue:
Colon (rectum)
Morphology:
Epithelial; monolayer, or clumping, some floating aggregates
Properties:
Adherent
Cytogenic data:
Tumorigenic.
Patient:
Male, Asian (Mongoloid), 52 yrs of age
Medium:
AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS
Subculture:
1:3 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 11, 12
D5S818: 10
D7S820: 10
THO1: 9
TPOX: 8, 11
vWA: 14, 17
Mutations:
Microsatellite instability: Stable (MSS)
Doubling time: 64 hours
codon 12, wild type
TP53 p.Arg273Leu (c.818G> T)
p53, exon 8;
p15, deleted Mutations in SNU-503 Cells
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: SNU-503 (AddexBio C0009015)
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Cell Lines