HCT-8 cells

Disease:
Ileocecal colorectal adenocarcinoma
Origin:
This cell line was derived from a 67 year-old male.
Species:
Homo sapiens
Tissue:
Colon
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
2n = 46, pseudodiploid
Patient:
67 year old male
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% horse serum
Subculture:
Subculture at 1:4; feed cells 3 to 4 times weekly. Culture at 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 12
D13S317: 8, 11
D16S539: 12, 13
D5S818: 13
D7S820: 10, 12, 11.3
THO1: 7, 9.3
TPOX: 8, 11
vWA: 18, 19
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: HCT-8 (AddexBio C0009017)
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Cell Lines