SW1116 cells

Disease:

Dukes' type A, grade III, colorectal adenocarcinoma

Origin:

Derived from a grade II adenocarcinoma of the colon extending into the muscularis from a 73 year old Caucasian male. The cells exhibit brush borders and produce high levels of CEA.

Species:

Homo sapiens

Tissue:

Colon

Morphology:

Epithelial;

Properties:

Adherent

Cytogenic data:

2n = 46, hypotriploid

Patient:

73 year old Caucasian, male

Medium:

AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBS (5% CO2 is not required)

Subculture:

Subculture at 1:3 to 1:6; feed cells 1 to 2 times weekly. Culture at 37C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO