CMT-93 cells

Disease:

Polyploid carcinoma, rectum carcinoma

Origin:

From a 19 month old male mouse (C57BL/1CRF) which had received an i.p. injection of MAMA each week for 18 months. 4th in vivo passage - source of explant culture. Cells form acini and junctional complexes and produce large tumours in nude mice (6 x 1, 000, 000 cells inoculum) after 1 month. Tumors developed in nude mice within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

Species:

Mus musculus, mouse

Tissue:

Rectum

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Hyperdiploid, modal no. 50

Patient:

19 month old male mouse (C57BL/1CRF)

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

When 80% confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:4 to 1:10; seed at 3x10^4 cells/cm2, feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO