CMT-93 cells

Disease:
Polyploid carcinoma, rectum carcinoma
Origin:
From a 19 month old male mouse (C57BL/1CRF) which had received an i.p. injection of MAMA each week for 18 months. 4th in vivo passage - source of explant culture. Cells form acini and junctional complexes and produce large tumours in nude mice (6 x 1, 000, 000 cells inoculum) after 1 month. Tumors developed in nude mice within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells
Species:
Mus musculus, mouse
Tissue:
Rectum
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Hyperdiploid, modal no. 50
Patient:
19 month old male mouse (C57BL/1CRF)
Medium:
AddexBio-formulated DMEM (C0003-01) + 10% FBS
Subculture:
When 80% confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:4 to 1:10; seed at 3x10^4 cells/cm2, feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: CMT-93 (AddexBio C0009020)
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Cell Lines