H716 cells

Disease:

Colorectal adenocarcinoma

Origin:

This line was derived from cells present in ascites fluid obtained from a patient after treatment with 5-fluorouracil.

Species:

Homo sapiens, human

Tissue:

Cecum

Morphology:

Epithelial

Properties:

Suspension, multicell aggregates and some adherent cells

Cytogenic data:

modal number = 61; range = 55 to 64. This is a hypotriploid human cell line. The modal chromosome number is 61, occurring in 28% of cells. The rate of higher ploidies is 1.2%. Twelve marker chromosomes are common in these cells. These include t(7q, 16q), t(5p, 7p), t(4q, 9q) and 9 others. A large number of DM's is found in every cell. The N21 is absent; and N9, N11, N13, N20, N22 and X are single-copied. No Y chromosome was detected in the Q-banded slide preparation.

Patient:

Male, Caucasian, 33 years of age

Medium:

AddexBio-formulated RPMI-1640 (C0004-01) + 10% FBS

Subculture:

Cultures can be maintained by the addition of fresh medium or replacement of medium Cultures can be established by centrifugation with subsequent resuspension at 4 to 8 x 10^5 viable cells/mL.. A subcultivation ratio of 1:3 to 1:6 is recommended. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO