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Esophageal carcinoma Origin: Established from the adenocarcinoma of the lower esophagus (Barrett's metaplasia), equivalent to OE33 Species:Homo sapiens Tissue:Esophagus Morphology:Epithelial Properties:Adherent Cytogenic data:express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice Patient:Female, Caucasian, 73 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
Established from the adenocarcinoma of the lower esophagus (Barrett's metaplasia), equivalent to OE33 Species:Homo sapiens Tissue:Esophagus Morphology:Epithelial Properties:Adherent Cytogenic data:express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice Patient:Female, Caucasian, 73 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
Homo sapiens Tissue:Esophagus Morphology:Epithelial Properties:Adherent Cytogenic data:express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice Patient:Female, Caucasian, 73 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
Esophagus Morphology:Epithelial Properties:Adherent Cytogenic data:express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice Patient:Female, Caucasian, 73 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
Epithelial Properties:Adherent Cytogenic data:express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice Patient:Female, Caucasian, 73 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
Adherent Cytogenic data:express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice Patient:Female, Caucasian, 73 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice Patient:Female, Caucasian, 73 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
Female, Caucasian, 73 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Related cells by Organ: EC-109 cells HKESC-1 cells OE33 cells JROECL33 cells NE-1 cells
EC-109 cells
HKESC-1 cells
OE33 cells
JROECL33 cells
NE-1 cells
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