JROECL33 cells

Established from the adenocarcinoma of the lower esophagus (Barrett's metaplasia), equivalent to OE33

Species:

Homo sapiens

Tissue:

Esophagus

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice

Patient:

Female, Caucasian, 73 years of age

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS

Subculture:

1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level: