A427 cells

Disease:
Lung carcinoma
Origin:
Established from the lung carcinoma of a 52-year-old Caucasian man
Species:
Homo sapiens
Tissue:
Lung
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
hypotriploid to hypertriploid with abnormalities
Patient:
Male, Caucasian, 52 yrs of age
Medium:
DMEM/F12 + 10% FBS
Subculture:
1:2 to 1:6 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level: I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Isoenzymes: AK-1, 2; ES-D, 1; G6PD, B; GLO-I, 1; PGM1, 1-2; PGM3, 1
DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 10, 12; D13S317: 11, 12; D16S539: 11, 13; D5S818: 12; D7S820: 8, 12; THO1: 9; TPOX: 8, 11; vWA: 17
If use of this culture results in a scientific publication, it should be cited in the publication as: A427 (AddexBio C0016004)
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Cell Lines