H520 cells

Disease:

Squamous cell carcinoma

Origin:

This cell line was derived from a sample of a lung mass taken from a patient with squamous cell carcinoma of the lung

Species:

Homo sapiens

Tissue:

Lung

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

This is a hypotriploid human cell line. The modal chromosome number is 58 occurring at 30%.

Patient:

Male

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS

Subculture:

When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:2 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO