H358 cells

Disease:
Bronchioalveolar carcinoma; non-small cell lung cancer
Origin:
This line was derived in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy.
Species:
Homo sapiens, human
Tissue:
Lung/bronchiole; derived from metastatic site: alveolus
Morphology:
Epithelial
Properties:
Adherent
Patient:
Male
Medium:
AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS
Subculture:
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37C. A subcultivation ratio of 1:3 to 1:6 is recommended. Renew medium once every 2 to 3 days depending on cell density.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 11, 12
D13S317: 8, 12
D16S539: 12, 13
D5S818: 10, 12
D7S820: 10, 11
THO1: 6
TPOX: 8, 9
vWA: 17
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: H358 (AddexBio C0016016)

Cell Lines