H1581 cells

Disease:

Stage 4, non-small cell lung cancer.

Origin:

This cell line was established in January of 1987.

Species:

Homo sapiens, human

Tissue:

Lung

Morphology:

Epithelial

Properties:

Mixed, adherent and suspension, large cells

Patient:

Caucasian, male, 44 years of age. The patient was a smoker, 75 pack years.

Medium:

DMEM:F12 1:1 Medium + 10% FBS

Subculture:

Cultures can be maintained by the addition of fresh medium. Cells grow mostly as floating aggregates of round cell clusters with some attached cells. Clusters are alive while single cells have very low viability. Attached cells can be shaken loose. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 5% (v/v) DMSO