H1373 cells

Disease:
Stage 3A, adenocarcinoma.
Origin:
The line was established in March 1986. The patient received prior chemotherapy and radiation therapy. The patient was a smoker, 30 packs year.
Species:
Homo sapiens, human
Morphology:
Epithelial-like
Properties:
Adherent
Cytogenic data:
Modal number = 60; range = 52-118; del(p14)
Patient:
Black, male, 56 years of age.
Medium:
AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS
Subculture:
Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 9, 10
D13S317: 6
D16S539: 10
D5S818:12
D7S820: 10, 12
THO1:8, 9.3
TPOX: 8, 10
vWA: 16
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: H1373 (AddexBio C0016047)

Cell Lines