H1373 cells

Disease:

Stage 3A, adenocarcinoma.

Origin:

The line was established in March 1986. The patient received prior chemotherapy and radiation therapy. The patient was a smoker, 30 packs year.

Species:

Homo sapiens, human

Morphology:

Epithelial-like

Properties:

Adherent

Cytogenic data:

Modal number = 60; range = 52-118; del(p14)

Patient:

Black, male, 56 years of age.

Medium:

AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO