SK-LU-1 cells

Disease:

Adenocarcinoma

Species:

Homo sapiens, human

Tissue:

Lung

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

The stemline chromosome number is hypotetraploid, with the 2S component occurring at 4.4%. Marker chromosomes 1p, t(1q; 11q); 11q+; t(13; ?); 16q+; t(12q; 18q); M10; t(2q; 13q); i(15); and ?t(xp; 21q) occurred in all S metaphases, and t(1p; ?); t(1p; 14q); t(16; ?), and t(14; 21) occurred in some. In addition, 4 to 9 small markers of unidentifiable origin occurred frequently. Chromosome No. 7 was generally hexasomic, X chromosomes were disomic, and normal No. 15 was absent. No Y chromosome was detected in the QM stained preparation.

Patient:

Caucasian, female, 46 years of age, the patient was a smoker at 30 pack year.

Medium:

AddexBio-formulated EMEM Medium (C0005-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:2 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO