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A2780cis cells

A2780cis cells

Manufacturer	Addexbio
Category	Cell Lines & Cell Culture Cell Lines
Type	Tumour Cells
Specific against	Human
Amount	One Frozen vial
Item no.	C0017003
eClass 6.1	32160890
eClass 9.0	32160890
Available
Biosafety Level	I
Cytogenic Data	Modal number: 46
DNA Profile	D5S818: 10, 12, 13; D13S317: 8, 9, 12; D7S820: 8, 9.3, 10.1, 11; D16S539: 10, 11, 12, 14; vWA: 14, 16, 19, 21; TH01: 6, 8; Amelogenin: X; TPOX: 8, 11; CSF1PO: 10, 14
Disease	Ovarian carcinoma
Freezing Medium	Complete culture medium supplemented with 5% (v/v) DMSO
Medium	AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS + 1 uM Cisplatin
Morphology	Epithelial
Origin	Derived from tumor tissue of an untreated patient. This cisplatin-resistant cell line has been developed by chronic exposure of the parent cisplatin-sensitive A2780 cell line (C0017002) to increasing concentrations of cisplatin. A2780cis is cross-resistant to melphalan, adriamycin and irradiation. An increased ability to repair DNA damage as well as cytogenetic abnormalities has been observed. In order to retain resistance cisplatinum has to be added to the media for every passage.
Pathogens	HIV: Negative, Hepatitis B: Negative, Hepatitis C: Negative
Patient	Not available
Properties	Adherent
Related Cells by Organ	A2780 cells, A2780cis cells, MDAH-2774 cells
Sterility	Bacteria: Negative, Yeast: Negative, Mycoplasma: Negative
Subculture	1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C. Cells will attach slowly after resuscitation and take up to 7 days to reach confluency. Recommendation: recover cells in media without cisplatin. Add after subculture of attached cells.
Tissue	Ovary
Storage condition	LN2
Manufacturers Category	Cell Lines

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Amount: One Frozen vial

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Delivery expected until 5/30/2024