ES-2 cells

Disease:

Clear cell carcinoma

Origin:

The ES-2 cell line was established from a surgical tumor specimen taken from a 47 year old black woman.

Species:

Homo sapiens, human

Tissue:

Ovary

Morphology:

Fibroblast-like

Properties:

Adherent

Cytogenic data:

This line has a complex hyperdiploid karyotype of 66XX to 88XX.

Patient:

Female, Black, 47 years of age.

Medium:

McCoy's 5a Medium + 10% FBS

Subculture:

Remove medium, and rinse with 0.05% trypsin, 0.03 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37C. A subcultivation ratio of 1:4 to 1:8 is recommended. Renew medium once every 2 to 3 days depending on cell density.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO