SK-UT-1 cells

Disease:
Grade III, mesodermal tumor (mixed); consistent with leiomyosarcoma.
Origin:
This cell line has little or no phosphorylated retinoblastoma protein. SK-UT-1 cells are capable of forming tumors when inoculated in immunocompromised mice.
Species:
Homo sapiens, human
Tissue:
Uterus
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
(P8) hypodiploid to hyperdiploid
Patient:
Caucasian; female; 75 years of age
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
Subculture sub-confluent cultures (70-80%) at 1:4 to 1:8 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 10, 11
D13S317: 13
D16S539: 13, 14
D5S818: 10, 11
D7S820: 9, 10
THO1:7
TPOX: 8
vWA: 15, 16
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: SK-UT-1 (AddexBio C0017007)

Cell Lines