SK-UT-1 cells

Disease:

Grade III, mesodermal tumor (mixed); consistent with leiomyosarcoma.

Origin:

This cell line has little or no phosphorylated retinoblastoma protein. SK-UT-1 cells are capable of forming tumors when inoculated in immunocompromised mice.

Species:

Homo sapiens, human

Tissue:

Uterus

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

(P8) hypodiploid to hyperdiploid

Patient:

Caucasian; female; 75 years of age

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:4 to 1:8 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 5% (v/v) DMSO