SK-OV-3 cells

Disease:
Ovarian serous cystadenocarcinoma
Origin:
Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumor. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells.
Species:
Homo sapiens, human
Tissue:
Ovary: ascites
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Hypodiploid to hypotetraploid
Patient:
Female, Caucasian, 64 years of age
Medium:
AddexBio-Formulated DMEM (C0003-03) + 10% FBS
Subculture:
Subculture sub-confluent cultures (85%) at 1:2 to 1:6 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
HPV: Negative
EBV: Negative
CMV: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 11
D13S317: 8, 11
D16S539: 12
D5S818:11
D7S820: 13, 14
THO1:9, 9.3
TPOX: 8, 11
vWA: 17, 18
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: SK-OV-3 (AddexBio C0017008)

Cell Lines