JavaScript seems to be disabled in your browser. You must have JavaScript enabled in your browser to utilize the functionality of this website.
Adenocarcinoma
The human pancreatic cancer cell line PSN1 was derived from pancreatic adenocarcinoma tissue. After transplantation into nude mice the PSN1 cell line was established from the xenograft. PSN1 cells are unique in that amplification of both c-myc (50-fold) and activated c-Ki-ras (3-6 fold) with a point mutation from GGT to CGT at codon 12 are present in the same degree as in the original tumour and contain increased amounts of c-myc and c-Ki-ras transcripts. Loss of one of the two p53 alleles and a point mutation from AAG to CAG at codon 132 in the remaining allele was also observed in PSN1.
Homo sapiens, human Tissue: Pancreas Morphology: Epithelial, epithelial-like Properties: Adherent Cytogenic data: PSN1 cells are unique in that amplification of both c-myc (50-fold) and activated c-Ki-ras (3-6 fold) with a point mutation from GGT to CGT at codon 12 are present in the same degree as in the original tumour and contain increased amounts of c-myc and c-Ki-ras transcripts. Loss of one of the two p53 alleles and a point mutation from AAG to CAG at codon 132 in the remaining allele was also observed in PSN1. Patient: Not Specified Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture: Subculture at a ratio of 1:2 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
Pancreas Morphology: Epithelial, epithelial-like Properties: Adherent Cytogenic data: PSN1 cells are unique in that amplification of both c-myc (50-fold) and activated c-Ki-ras (3-6 fold) with a point mutation from GGT to CGT at codon 12 are present in the same degree as in the original tumour and contain increased amounts of c-myc and c-Ki-ras transcripts. Loss of one of the two p53 alleles and a point mutation from AAG to CAG at codon 132 in the remaining allele was also observed in PSN1. Patient: Not Specified Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture: Subculture at a ratio of 1:2 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
Epithelial, epithelial-like Properties: Adherent Cytogenic data: PSN1 cells are unique in that amplification of both c-myc (50-fold) and activated c-Ki-ras (3-6 fold) with a point mutation from GGT to CGT at codon 12 are present in the same degree as in the original tumour and contain increased amounts of c-myc and c-Ki-ras transcripts. Loss of one of the two p53 alleles and a point mutation from AAG to CAG at codon 132 in the remaining allele was also observed in PSN1. Patient: Not Specified Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture: Subculture at a ratio of 1:2 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
Adherent Cytogenic data: PSN1 cells are unique in that amplification of both c-myc (50-fold) and activated c-Ki-ras (3-6 fold) with a point mutation from GGT to CGT at codon 12 are present in the same degree as in the original tumour and contain increased amounts of c-myc and c-Ki-ras transcripts. Loss of one of the two p53 alleles and a point mutation from AAG to CAG at codon 132 in the remaining allele was also observed in PSN1. Patient: Not Specified Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture: Subculture at a ratio of 1:2 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
PSN1 cells are unique in that amplification of both c-myc (50-fold) and activated c-Ki-ras (3-6 fold) with a point mutation from GGT to CGT at codon 12 are present in the same degree as in the original tumour and contain increased amounts of c-myc and c-Ki-ras transcripts. Loss of one of the two p53 alleles and a point mutation from AAG to CAG at codon 132 in the remaining allele was also observed in PSN1. Patient: Not Specified Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture: Subculture at a ratio of 1:2 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
Not Specified Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture: Subculture at a ratio of 1:2 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture: Subculture at a ratio of 1:2 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
Subculture at a ratio of 1:2 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
Complete growth culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
Amelogenin: X CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
CSF1PO: 12 D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
D13S317: 10 D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
D16S539: 10, 11 D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
D5S818: 11, 13 D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
D7S820: 10 THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
THO1:6 TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
TPOX: 8, 11 vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
vWA: 17 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
If use of this culture results in a scientific publication, it should be cited in the publication as: PSN-1 (AddexBio C0018013)
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
Request Data Sheet
Request product datasheet
Request safety datasheet
Compare
Add to wishlist
Get an offer
Request delivery time
Ask a technical question
Submit a bulk request
sales@hoelzel.de
« Back
Subscribe, get 15% off every fifth order and have your items delivered on time!
Forgot Your Password?
Not yet registered? Create account here!