C32 cells

Disease:
Amelanotic melanoma
Origin:
C32 belongs to the C32r type cultures with the marker M2r substituting the original ring marker M2R. Tumours can be produced in nude mice after 1-2 months (1, 000, 000 cells injected).
Species:
Homo sapiens
Tissue:
Skin
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Tumorigenic; Modal no. 45.
Patient:
Male, Caucasian, 53 yrs of age
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR): Amelogenin: X, Y ; CSF1PO: 11, 12; D13S317: 12; D16S539: 11, 12; D5S818: 12; D7S820: 9, 11; THO1: 9.3; TPOX: 9; vWA: 14, 19; Mutations in C32 Cells
Related cells by Organ: A431 cells
SK-Mel-28 cells
UACC-62 cells
C-32 cells
HaCaT cells

Cell Lines