Disease:
Gastric carcinoma Origin:
Derived from metastatic pleural effusion; supraclavicular and axillary lymph nodes and Douglas cul-de-sac Species:
Homo sapiens Tissue:
Stomach Morphology:
Sperical Properties:
Mixed Suspension and Adherent for long term culture Cytogenic data:
Tumorigenic; The stemline chromosome number is hypotetraploid Patient:
Male, Asian, 55 yrs of age Medium:
IMDM + 20% FBS Subculture:
1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression:
Isoenzymes:
AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR):
Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells