Y79 cells

Disease:

Retinoblastoma

Origin:

The Y79 line was isolated by explant culture of a primary tumor from the right eye obtained immediately after enucleation in 1971.

Species:

Homo sapiens

Tissue:

Eye, retina

Morphology:

Multiple cell clusters

Properties:

Suspension

Cytogenic data:

hypertriploidy, with abnormalities including dicentrics, breaks, pulverizations and minutes

Patient:

Female, 2.5 yrs of age, Caucasian

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS

Subculture:

Seed at 2.5x10^5 cells/mL, maintain at 1.0 x 10^6 cells/ml; feed cells twice-weekly, split of ca. 1:2 to 1:4. Culture at 5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 10% (v/v) DMSO