Y79 cells

Disease:
Retinoblastoma
Origin:
The Y79 line was isolated by explant culture of a primary tumor from the right eye obtained immediately after enucleation in 1971.
Species:
Homo sapiens
Tissue:
Eye, retina
Morphology:
Multiple cell clusters
Properties:
Suspension
Cytogenic data:
hypertriploidy, with abnormalities including dicentrics, breaks, pulverizations and minutes
Patient:
Female, 2.5 yrs of age, Caucasian
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS
Subculture:
Seed at 2.5x10^5 cells/mL, maintain at 1.0 x 10^6 cells/ml; feed cells twice-weekly, split of ca. 1:2 to 1:4. Culture at 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 10% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X, X
CSF1PO: 11, 12
D13S317: 11, 12
D16S539: 13, 14
D5S818: 11, 12
D7S820: 8, 9
THO1: 6.9, 3
TPOX: 8
vWA: 15, 18

Cell Lines