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The line was formed by fusing BALB/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma. The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 ug/ml and are HAT sensitive.
Mus musculus, mouse Morphology: Lymphoblast-like; hybridoma, B lymphocyte Properties: Suspension Strain: BALB/c Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Maintain cell density between 5x10^4 and 5x10^5 viable cells/mL. Some cells can attach and be transferred by shaking them loose. Culture at 5% CO2, 37C. Renew medium once every 2 to 4 days depending on cell density. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sp2/0-Ag14 (AddexBio C0033005)
Lymphoblast-like; hybridoma, B lymphocyte Properties: Suspension Strain: BALB/c Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Maintain cell density between 5x10^4 and 5x10^5 viable cells/mL. Some cells can attach and be transferred by shaking them loose. Culture at 5% CO2, 37C. Renew medium once every 2 to 4 days depending on cell density. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sp2/0-Ag14 (AddexBio C0033005)
Suspension Strain: BALB/c Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Maintain cell density between 5x10^4 and 5x10^5 viable cells/mL. Some cells can attach and be transferred by shaking them loose. Culture at 5% CO2, 37C. Renew medium once every 2 to 4 days depending on cell density. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sp2/0-Ag14 (AddexBio C0033005)
BALB/c Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Maintain cell density between 5x10^4 and 5x10^5 viable cells/mL. Some cells can attach and be transferred by shaking them loose. Culture at 5% CO2, 37C. Renew medium once every 2 to 4 days depending on cell density. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sp2/0-Ag14 (AddexBio C0033005)
AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Maintain cell density between 5x10^4 and 5x10^5 viable cells/mL. Some cells can attach and be transferred by shaking them loose. Culture at 5% CO2, 37C. Renew medium once every 2 to 4 days depending on cell density. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sp2/0-Ag14 (AddexBio C0033005)
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell density between 5x10^4 and 5x10^5 viable cells/mL. Some cells can attach and be transferred by shaking them loose. Culture at 5% CO2, 37C. Renew medium once every 2 to 4 days depending on cell density. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sp2/0-Ag14 (AddexBio C0033005)
Complete culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sp2/0-Ag14 (AddexBio C0033005)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sp2/0-Ag14 (AddexBio C0033005)
Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sp2/0-Ag14 (AddexBio C0033005)
Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sp2/0-Ag14 (AddexBio C0033005)
If use of this culture results in a scientific publication, it should be cited in the publication as: Sp2/0-Ag14 (AddexBio C0033005)
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
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