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Carcinoma, Cancer
Spleen cells were fused with P3X63Ag8 myeloma cells.
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) Tissue: Hybridoma; B lymphocyte Morphology: Lymphoblast Properties: Suspension Clinical Data: The antibody binds to human tumors of endodermal origin (gastric, pancreatic and colorectal carcinomas) and detects the antigen in serum and urine of cancer patients. Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days depending on cell density. Freezing Medium:Complete growth medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: 1116-NS-19-9 (AddexBio C0033006)
Hybridoma; B lymphocyte Morphology: Lymphoblast Properties: Suspension Clinical Data: The antibody binds to human tumors of endodermal origin (gastric, pancreatic and colorectal carcinomas) and detects the antigen in serum and urine of cancer patients. Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days depending on cell density. Freezing Medium:Complete growth medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: 1116-NS-19-9 (AddexBio C0033006)
Lymphoblast Properties: Suspension Clinical Data: The antibody binds to human tumors of endodermal origin (gastric, pancreatic and colorectal carcinomas) and detects the antigen in serum and urine of cancer patients. Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days depending on cell density. Freezing Medium:Complete growth medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: 1116-NS-19-9 (AddexBio C0033006)
Suspension Clinical Data: The antibody binds to human tumors of endodermal origin (gastric, pancreatic and colorectal carcinomas) and detects the antigen in serum and urine of cancer patients. Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days depending on cell density. Freezing Medium:Complete growth medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: 1116-NS-19-9 (AddexBio C0033006)
The antibody binds to human tumors of endodermal origin (gastric, pancreatic and colorectal carcinomas) and detects the antigen in serum and urine of cancer patients. Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days depending on cell density. Freezing Medium:Complete growth medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: 1116-NS-19-9 (AddexBio C0033006)
AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days depending on cell density. Freezing Medium:Complete growth medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: 1116-NS-19-9 (AddexBio C0033006)
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days depending on cell density. Freezing Medium:Complete growth medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: 1116-NS-19-9 (AddexBio C0033006)
Complete growth medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: 1116-NS-19-9 (AddexBio C0033006)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: 1116-NS-19-9 (AddexBio C0033006)
Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: 1116-NS-19-9 (AddexBio C0033006)
Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: 1116-NS-19-9 (AddexBio C0033006)
If use of this culture results in a scientific publication, it should be cited in the publication as: 1116-NS-19-9 (AddexBio C0033006)
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