BV-173

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human
Amount	1 cryovial
Item no.	CLS-300133
eClass 6.1	32160890
eClass 9.0	32160890
Available
Manufacturers Category	Leukemia cell lines
Tissue	Blood
Disease	Chronic myeloid leukemia
Age	45 years
Gender	Male
Ethnicity	Caucasian
Cell Type	Undifferentiated blast cells
Growth Properties	Suspension
Biosafety Level	1
Reverse Transcriptase	Negative (ELISA)
Ploidy Status	t(9, 22) Modal Number: 2n=46
Mutational Profile	b2a2 BCR-ABL
Culture Medium	RPMI 1640
Medium Supplements	10% FBS, 2 mM L-glutamine
Doubling Time	35 hours
Subculturing	Start new cultures at 1 x 10^5 viable cells/ml. Subculture when the cell concentration has reached 1 x 10^6 cells/ml. Prepare dilutions by transferring an appropriate volume of cell suspension into new flasks containing fresh cell culture medium. Optimal cell growth at 0.5 - 2 x 10^6 cells/ml.
Split Ratio	A ratio of 1:3 is recommended
Seeding Density	1 x 10^5 cells/ml
Fluid Renewal	2 to 3 times per week
Freezing Recovery	Allow the cells to recover from the freezing process for at least 48 hours.
Freeze Medium	CM-1 (Catalog number CLS-800650)
Amelogenin	X, X
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CLS-300133-datasheet.pdf

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Amount: 1 cryovial

available

Delivery expected until 5/23/2024