HNO210 Cells

The HNO210 cell line is derived from a laryngeal squamous cell carcinoma, a subtype of head and neck squamous cell carcinoma (HNSCC). This cell line has been extensively characterized for its genetic and molecular features, making it a valuable model for studying the pathogenesis and treatment responses of HNSCC. Chromosomal comparative genomic hybridization (cCGH) analysis of HNO210 has revealed several significant chromosomal aberrations. Notably, it exhibits DNA copy number gains in chromosomal regions 3q, 7p, 7q, 9p, 9q, 20p, and 20q, and copy number losses in 3p, 4p, 4q, and chromosome 21. These genetic alterations are common in HNSCC and are associated with aggressive tumor behavior and poor patient prognosis. In particular, the amplification of regions such as 3q and 11q13, which is seen in many HNSCC cell lines, is of interest due to its correlation with increased expression of oncogenes such as CCND1 (cyclin D1) and CTTN (cortactin). These genes are involved in cell cycle regulation and cytoskeletal organization, respectively, and their overexpression can contribute to enhanced cell proliferation, invasion, and metastasis. The HNO210 cell line, with its distinct genetic profile, serves as a robust model for investigating the molecular mechanisms underlying laryngeal cancer progression and for testing targeted therapies that address these specific genetic abnormalities. Additionally, this cell line is part of a panel used to explore the efficacy of combination therapies, such as the use of cisplatin with thalidomide, which have shown promise in enhancing anti-tumor activity in vitro and in vivo. This makes HNO210 not only crucial for basic cancer research but also for translational studies aimed at improving therapeutic outcomes for patients with HNSCC.

Monolayer, adherent

69 years

Caucasian

1

Supplement the medium with 10% FBS

2 to 3 times per week

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

An initial ratio of 1:3 is recommended according to the growth rate