2427T Cells

Originating from a primary tumor of a 64-year-old female Caucasian patient diagnosed with lung squamous cell carcinoma, 2427T provides a valuable in vitro model that recapitulates the morphological traits of the original tumor tissue. Characterized by their distinctive small, round shape and propensity to aggregate into clusters, 2427T cells exhibit key morphological features typical of squamous cell carcinoma (SCC).
A defining characteristic of the 2427T cell line is its expression of cytokeratin 5/6 (CK5/6), a marker indicative of its SCC origin. The heterogeneous expression of CK5/6 hints at the presence of diverse cell subpopulations within the 2427T culture, presenting an opportunity for further exploration of intratumoral heterogeneity.
Immunophenotyping of 2427T has revealed its unique profile, including the lack of adenocarcinoma-associated marker CK7, hemato-endothelial progenitor marker CD34, and leukocyte marker CD45, reinforcing its classification within the squamous lineage. Interestingly, while the cell line generally shows negativity for neuroendocrine markers such as CD56, synaptophysin (SYP), neuron-specific enolase (NSE), and chromogranin A (CHGA), the expression of SYP in a subset of cells suggests a degree of neuroendocrine marker heterogeneity.
Crucially, the 2427T cell line does not harbor mutations in EGF-R or k-ras, distinguishing it from other models and underscoring its potential as a novel resource for delving into the biology and therapeutic vulnerabilities of squamous cell non-small cell lung cancer (NSCLC). This absence of common oncogenic mutations positions 2427T as an invaluable tool for research aimed at uncovering the underlying mechanisms of squamous cell carcinoma pathogenesis and progression.

CLS Cell Lines Service

DMEM + Hams F12

9606

Adherent

64 years

Female

Small round, show a high nucleus-to-cytoplasm ratio and grow

Supplement the medium with 10% FBS

CM-1 (Cytion catalog number 800100)

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.

Q54583507