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LNCaP Cells

LNCaP Cells

Für die Verwendung dieses Produktes fordert der Hersteller das zu Ihrer Institution passende, ausgefüllte und unterschriebene Formular:

Profit Organisation/Nutzung: CLS_CLEAR.pdf
Non-Profit Organisation/Nutzung: Non_Profit_CLS_Supply_Agreement.pdf

Bitte laden Sie dieses entweder beim Kaufabschluss hoch oder senden es alternativ an die purchasing@hoelzel.de

Item no.	CLS-300265
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Certificate	For a certificate of analysis, please send an inquiry to info@hoelzel.de.
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	LNCAP,LNCap,Ln-Cap,Lymph Node Carcinoma of the prostate
Available
Manufacturer - Category	Prostate cancer cell lines
Description	LNCaP cells, derived from a metastatic lesion in a lymph node of a prostate cancer patient, represent a critical tool in prostate cancer research, particularly for studying the role of androgens and androgen receptor (AR) dynamics in cancer progression. The LNCaP cell line is characterized by their androgen-sensitive growth and offers a window into the mechanisms underlying prostate cancer's response to hormonal manipulation. As a model for metastatic prostate cancer, parental LNCaP cells and their sublines, such as the LNCaP clone FGC, provide clinically relevant insights into disease progression, especially in the context of metastasis to bone, forming osteoblastic lesions akin to those observed in human prostate cancer. The LNCaP human prostate cancer cell line expresses a mutated form of the AR gene with broader steroid-binding specificity and therefore is pivotal for understanding the complex interplay between AR activity and prostate cancer progression. This includes the examination of AR downstream targets like PSA and NKx3.1, which are crucial for prostatic epithelial cell function. LNCaP cells are further used in cytotoxicity studies such as those induced by ripl or the potential therapeutic effects of compounds like amygdalin, within the scope of intracellular drug delivery strategies. In summary, the human prostate carcinoma cell line LNCaP serves as a cornerstone in understanding the role of androgens in cancer progression and prostate cancer, offering insights into hormone-responsive cancers, the challenges of resistant prostate cancer, and the potential for therapeutic interventions. The LNCaP cell line is considered one of the classic and most widely used human prostate cancer cell lines, alongside DU145 and PC3 cells.
Tissue	Prostate
Growth properties	Adherent, clusters
Disease	Carcinoma
Age	50 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Biosafety Level	1
Culture Medium	EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c)
Medium Supplements	Supplement the medium with 10% FBS
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid Renewal	Every 3 days
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-1 (Cytion catalog number 800100)
Handling of Cryopreserved Cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions	When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer	Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty	We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Amelogenin	X, Y
Tumorigenic	Yes, in nude mice
Split Ratio	A ratio of 1:3 to 1:6 is recommended
Seeding Density	1 to 2 x 10^4 cells/cm^2
Metastatic Site	Left supraclavicular lymph node
Doubling Time	60 hours
Products	human prostatic acid phosphatase, prostate specific antigen
Protein Expression	p53 positive
Karyotype	pseudodiploid male, seven marker chromosomes, modal number = 46, range = 33 to 91
Receptors Expressed	Androgen, estrogen

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

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Delivery expected until 10/30/2025

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