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LXF-289 Cells

LXF-289 Cells

Für die Verwendung dieses Produktes fordert der Hersteller das zu Ihrer Institution passende, ausgefüllte und unterschriebene Formular:

Profit Organisation/Nutzung: CLS_CLEAR.pdf
Non-Profit Organisation/Nutzung: Non_Profit_CLS_Supply_Agreement.pdf

Bitte laden Sie dieses entweder beim Kaufabschluss hoch oder senden es alternativ an die purchasing@hoelzel.de

Item no.	CLS-300269
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Certificate	For a certificate of analysis, please send an inquiry to info@hoelzel.de.
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	LxF289,LxF 289,LxF 289L
Available
Manufacturer - Category	Lung cancer cell lines
Description	The LxF-289 cell line is a human lung adenocarcinoma cell line established from a 63-year-old male patient. This cell line has a doubling time of approximately 50 hours, making it suitable for studies that require consistent cell proliferation. LxF-289 is particularly valuable in research focused on lung cancer, especially non-small cell lung cancer (NSCLC), as it provides a robust in vitro model for studying the molecular mechanisms underlying cancer progression, treatment resistance, and the effects of therapeutic interventions. Studies on LxF-289 have demonstrated that this cell line exhibits characteristics that make it responsive to specific genetic and therapeutic manipulations. For instance, research has shown that LxF-289, along with other lung cancer cell lines, can undergo significant cell death when treated with an adenovirus expressing antisense heat shock protein 70 (Hsp70). This cell death is p53-independent and does not require DNA cleavage, suggesting that Hsp70 plays a crucial role in the survival of lung cancer cells. Notably, this response is selective to cancer cells, as normal lung fibroblasts and bronchial epithelial cells do not show similar levels of cytotoxicity when Hsp70 is downregulated, highlighting the potential of targeting Hsp70 in lung cancer therapy. Moreover, LxF-289 has been used to study the effects of irradiation on drug resistance-related proteins. The cell line exhibited overexpression of glutathione S-transferase (GSTπ) at both mRNA and protein levels following irradiation. This overexpression is associated with the development of multidrug resistance, which is a significant challenge in the clinical management of lung cancer. These findings underscore the utility of LxF-289 in exploring the mechanisms of resistance and testing novel strategies to overcome it.
Tissue	Lung
Growth properties	Adherent
Disease	Adenocarcinoma
Age	62 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Biosafety Level	1
Culture Medium	RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Medium Supplements	Supplement the medium with 10% FBS
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid Renewal	Every 3 to 5 days
Freezing Recovery	24 to 48 hours
Freeze Medium	CM-1 (Cytion catalog number 800100)
Handling of Cryopreserved Cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions	When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer	Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty	We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Tumorigenic	Yes, in nude mice
Split Ratio	A ratio of 1:2 to 1:6 is recommended
Seeding Density	1 x 10^4 cells/ml
Reverse Transcriptase	negative

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

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Delivery expected until 10/30/2025

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