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SaOS-2 Cells

SaOS-2 Cells

Für die Verwendung dieses Produktes fordert der Hersteller das zu Ihrer Institution passende, ausgefüllte und unterschriebene Formular:

Profit Organisation/Nutzung: CLS_CLEAR.pdf
Non-Profit Organisation/Nutzung: Non_Profit_CLS_Supply_Agreement.pdf

Bitte laden Sie dieses entweder beim Kaufabschluss hoch oder senden es alternativ an die purchasing@hoelzel.de

Item no.	CLS-300331
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Certificate	For a certificate of analysis, please send an inquiry to info@hoelzel.de.
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	SAOS-2,Saos-2,SAOS 2,Saos 2,Saos2,SaOs2,SAOS2,Sarcoma OSteogenic-2,SaOS,SAOS
Available
Manufacturer - Category	Bone cancer cell lines
Description	Saos-2 cells are an osteosarcoma cell line derived from the primary osteogenic sarcoma of an 11-year-old Caucasian female. These cells are a widely recognized model for studying osteosarcoma and bone biology, due to their osteoblastic characteristics and the ability to produce a bone-like extracellular matrix. Characterized by their high level of alkaline phosphatase activity and expression of bone-specific proteins such as osteocalcin and osteopontin, Saos-2 cells serve as an effective in vitro system to study bone formation and the pathophysiology of osteosarcoma. They are particularly valuable for investigating cellular responses to various biochemical stimuli and mechanical forces that mimic the bone environment. Saos-2 cells also exhibit an aneuploid karyotype, lacking several chromosomes but with extra copies of others, typical of many cancer cell lines. They are negative for mycoplasma and possess a robust capacity for calcification, making them suitable for assays related to mineral deposition. In the context of cancer research, Saos-2 cells are extensively used to explore the molecular mechanisms of tumorigenesis, metastasis, and the effects of anticancer drugs on osteosarcoma. The cells are also employed to study gene expression profiles associated with osteoblastic differentiation and malignancy. Due to their high transfectability, Saos-2 cells are amenable to genetic manipulation, which allows for the study of gene function and the validation of molecular targets for therapeutic intervention. This adaptability has facilitated significant advancements in understanding the genetic and molecular basis of bone cancer and in developing targeted treatments for osteosarcoma.
Tissue	Bone
Growth properties	Monolayer, adherent
Disease	Osteosarcoma
Age	11 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Biosafety Level	1
Culture Medium	DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Medium Supplements	Supplement the medium with 10% FBS
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid Renewal	2 to 3 times per week
Freezing Recovery	Fast
Freeze Medium	CM-1 (Cytion catalog number 800100)
Handling of Cryopreserved Cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions	When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer	Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty	We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Tumorigenic	No
Split Ratio	A ratio of 1:2 to 1:4 is recommended
Seeding Density	1 x 10^4 cells/cm^2
Doubling Time	35 to 40 hours
Antigen Expression	Blood Type B, Rh+, HLA A2, A3, Bw16, Bw47
Karyotype	The stemline chromosome number is hypotriploid with the modal number of 56 chromosomes per cell and the 2S component occurring at 13.2%. Over two-thirds of the chromosome complement consisted of structurally rearranged chromosomes. Most marker chromosomes had complex rearrangements. The origin of the segments composing these markers could not be identified. Of the identifiable markers, 6p+/q+, 7p+, 11p+, and 12p+ occasionally were present at 2 copies per cell. The Y chromosome was not detected in the QM stained preparation.
Receptors Expressed	Epidermal growth factor (EGF), transforming growth factor beta (type 1 and type 2)
Msi Status	Stable (MSS)
Isoenzymes	Me-2, 1, PGM3, 1-2, PGM1, 1-2, ES-D, 2, AK-1, 1, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0002

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

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Delivery expected until 10/30/2025

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