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SK-BR-3 Cells European Partner

Für die Verwendung dieses Produktes fordert der Hersteller das zu Ihrer Institution passende, ausgefüllte und unterschriebene Formular: Bitte laden Sie dieses entweder beim Kaufabschluss hoch oder senden es alternativ an die purchasing@hoelzel.de
Item no. CLS-300333
Manufacturer CLS Cell Lines Service
Amount 1 cryovial
Category
Type Cell line
Certificate For a certificate of analysis, please send an inquiry to info@hoelzel.de.
Specific against Human (Homo sapiens)
Dry ice Yes
ECLASS 10.1 42040401
ECLASS 11.0 42040401
UNSPSC 41106509
Alias SK-Br-3,Sk-Br-3,SK BR 03,SKBR-3,SKBr-3,SK-BR3,SKBr3,SkBr3,SKBR3
Available
Manufacturer - Category
Breast cancer cell lines
Description
SK-BR-3 cells are a human breast cancer cell line isolated from the pleural effusion of a 43-year-old female patient with metastatic breast cancer. SKBR3 cells were established in the early 1970s and are known for their overexpression of the human epidermal growth factor receptor 2 (HER2), a receptor tyrosine kinase that plays a critical role in the pathogenesis and progression of certain types of breast cancer.
The cell line is characterized by genetic aberrations common in breast cancer, including amplification of the HER2 gene and mutations in the p53 tumor suppressor gene. The overexpression of HER2 in SK-BR-3 cells makes them a valuable model for studying HER2-positive breast cancer, which is characterized by aggressive growth and a poor prognosis, and for HER2-targeted therapies. SK-BR-3 cells have been instrumental in the study of trastuzumab (Herceptin), a monoclonal antibody against HER2 that has become a cornerstone in the treatment of HER2-positive breast cancer.
SK-BR-3 cells exhibit a robust in vitro growth rate and have been used in a variety of experimental setups, including studies on cell signaling, drug resistance, apoptosis, and the cancer cell cycle. These cells are also a key resource for the production of monoclonal antibodies and for research into the immune response to breast cancer cells.
In summary, the SK-BR-3 cell line is an indispensable tool in breast cancer research, offering profound insights into the biology of HER2-positive tumors and facilitating the development of targeted therapies that have significantly improved the outlook for patients with this challenging form of cancer.
Tissue
Breast, mammary gland
Growth properties
Monolayer, adherent
Disease
Invasive ductal carcinoma
Age
43 years
Gender
Female
Ethnicity
Caucasian
Morphology
Epithelial-like
Biosafety Level
1
Culture Medium
McCoys 5a, w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3 (Cytion article number 820200a)
Medium Supplements
Supplement the medium with 10% FBS
Subculturing
Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid Renewal
2 to 3 times per week
Freezing Recovery
After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium
Handling of Cryopreserved Cultures

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility
Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions
When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer
Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty
We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.
Amelogenin
X, X
Tumorigenic
Yes, in nude mice, forms poorly differentiated adenocarcinoma
Split Ratio
A ratio of 1:2 to 1:4 is recommended
Seeding Density
Start culture from cryovial at 3 x 10^4 cells/cm^2. Use 2 x 10^4 cells/cm^2 for continued subcultures
Metastatic Site
Pleural effusion
Doubling Time
30 hours
Antigen Expression
Blood Type A, Rh+, HLA A11, Bw22(+/-), B40, B18
Mutational Profile
TP53 mut
Protein Expression
p53 positive
Karyotype
(P9) hypertriploid to hypotetraploid (+A, +B, +C, +E, +F, +G, -D) with abnormalities including dicentrics, acrocentric fragments, rings, secondary constrictions, large metacentrics or polycentrics and large submetacentric marker
Isoenzymes
PGM3, 1, PGM1, 1-2, ES-D, 1, AK-1, 1-2, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0044

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 cryovial
Available: In stock
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